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KMID : 0613820180280050509
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Journal of Life Science
2018 Volume.28 No. 5 p.509 ~ p.516
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Inhibitory Effects of Flavonoids Isolated from the Leaves of Stewartia koreana on Nitric-oxide Production in LPS-stimulated RAW 264.7 Cells
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Lee Seung-Su
Band Myun-Ho
Park Se-Ho
Chung Dae-Kyun
Yang Seun-Ah
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Abstract
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Five phenolic compounds were isolated from the ethyl acetate fraction of leaves from Stewartia koreana, and their nitric-oxide (NO) inhibitory activities were measured to identify the major active constituents responsible for the efficacy of the extract against inflammatory reactions. These five compounds were quercetin (1), quercitrin (2), hyperin (3), quercetin-3-O-(6''-O-galloyl)-β-D-galactopyranoside (4), and kaempferol 3-O-[2'',6''-di-O-(trans-p-coumaroyl)]-β-D-glucopyranoside (5). Among the separated compounds in the EtOAc fraction, compounds 4 and 5 were isolated for the first time, and no study has yet reported their anti-inflammatory effects. The compounds were identified by spectroscopic analysis, and the isolated compounds showed significant NO inhibitory effects in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Compound 5 showed the most potent inhibitory effect (63.35% inhibition) against LPS-induced NO production compared to that of compound 1 (17.17%), compound 2 (5.0%), compound 3 (3.92%), and compound 4 (6.32%) at 10 μg/ml concentration. NO production was inhibited by suppressing the protein expression of inducible nitric-oxide synthase in LPS-stimulated RAW 264.7 macrophages. These results indicate that kaempferol 3-O-[2'',6''-di-O- (trans-p-coumaroyl)]-β-D-glucopyranoside might be the major active compound responsible for the anti-inflammatory effects of S. koreana.
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KEYWORD
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Glucopyranoside, kaempferol-3-o-[2'',6''-di-o-(trans-p-coumaroyl)]-β-D-, macrophage, nitric oxide, phenolic compounds, Stewartia koreana Nakai
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